Bacterial Transformation Lab Report Abstract

Conjugation transduction and transformation.

Bacterial transformation lab report abstract. This lab uses transformation to insert a specific gene into a plasmid so that the cell takes on those characteristics for which the gene codes rather it be resistance of ampicillin or kanamycin. It is when free floating genetic material in the cells environment travels across the cell membrane and alters its genes. Bacterial transformation lab report. Transformation involves the transfer of genetic information into a cell by directly taking up the dna.

The experiments purpose was to see if we could genetically manipulate the ecoli bacteria and maintain the glowing dna and become resistant to ampicylin which kills bacteria. The resent research and studies supports that we can genetically manipulate the ecoli because in the past this would change the cell function. Abstract the technique of transforming cells such as bacteria in genetic is pertinent for the improvements of molecular biology. Christine stevenson performed by.

This gene is called the gfp gene. Bacterial transformation lab procedures in our lab book. Coli so if e. Coli cells contain the ampicillin resistance gene the cells can survive exposure to ampicillin since the ampicillin resistance gene encodes an enzyme that inactivates the antibiotic.

To perform genetic transformation using the bacterial transformation method and to observe the results of bacterial. Coli so if e. When bacteria is transformed with genes that provide. Bacterial transformation is the easiest type of genetic transformation to create in a lab due to the single celled nature of bacteria.

This experiment was performed to test the hypothesis if lb nutrient broth pglo and pglo ampicillin and arabinose was placed in the e. Ampicillin is an antibiotic that kills e. Bacterial transformation lab ta. Abstract genetic transformation occurs when an organism is modified by the introduction of new genetic information which is incorporated into the organisms genome.

The objective of this experiment is to conduct bacterial transformation on e. The experiment conducted in this lab focuses on transformation. Lab report on bacterial transformation. Bacterial transformation lab report elizabeth amanquah genetics 311 monday 200 450 jan trybula abstract this study was undertaken to develop an understanding of bacterial transformation by plasmid dna.

The plasmid pglo contains an antibiotic resistance gene ampr and the gfp gene is regulated by the control region of the ara operon. The process for this experiment came from the investigation 8 biotechnology. Coli plates then there will be a significant growth in the newly transformed bacteria and it will possess the ability to glow under uv light. The objectives of this lab are.

Coli bacteria by successfully inserting a plasmid pglo and getting the bacteria to express a gene that allows it to glow under uv light. This study enables one to observe various traits displayed by transformed bacterial cells.